Short pulse cytochalasin B treatment of mouse fibroblasts.
نویسندگان
چکیده
منابع مشابه
Cytochalasin B and the Adenosine Triphosphate Content of Treated Fibroblasts
INTRODUCTION the addition of the drug . Concentrations of about 3 μg/ml were required to observe altered cell morphology. The possibility was considered that depolymerization of microfilaments-with a resulting change in cell shape and function--resulted from a rapid depletion of intracellular energy levels brought about by the inhibition of sugar transport . The effects of cytochalasin B and io...
متن کاملInduction of triploidy in the mouse by cytochalasin B.
Mouse eggs fertilized in vivo were treated with cytochalasin B in vitro (5 mug/ml of culture medium) at the moment of extrusion of the second polar body (2-5, 3-0, 3-5 h after copulation). Cytochalasin B inhibits cytokinesis of the second maturation division, so that triploid digynic eggs are formed in over 50% of treated eggs. Triploid eggs were transplanted to the oviducts of recipients. On t...
متن کاملCytochalasin B
Cytochalasin B competitively inhibits the transport of uridine and thymidine by Novikoff rat hepatoma cells growing in suspension culture with apparent K(i)'s of 2 and 6 microM, respectively, but has no effect on the intracellular phosphorylation of the nucleosides. Choline transport is not affected by cytochalasin B. Results from pulse-chase experiments indicate that cytochalasin B has no dire...
متن کاملCytochalasin B
Cytochalasin B, a mold metabolite, has been shown to inhibit cytokinesis and motility (3), morphogenie development (18), endocytosis (5), and the transport of sugars (8-10, 12). I t has also been demonstrated that this drug inhibits the stimuatcd elaboration of extracellular substances from glands-the release of thyroxine from the thyroid gland after stimulation by thyroid-stimulating hormone (...
متن کاملLatent effects on in vitro development following cytochalasin B treatment of 8-cell mouse embryos.
Eight-cell mouse embryos when treated with 4.0 microgram/ml cytochalasin B (CB) in vitro undergo a reversible developmental arrest. Upon rinsing of embryos and subsequent culture in control medium, normal morphogenetic processes such as compaction of 8-cell embryos, cavitation, and post-blastocyst attachment and outgrowth are restored. However, the effects of CB on mouse embryos are not complet...
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ژورنال
عنوان ژورنال: CYTOLOGIA
سال: 1979
ISSN: 0011-4545,1348-7019
DOI: 10.1508/cytologia.44.175